For research needs, RT‐qPCR is typically used not simply to find out the existence or absence of an RNA but to measure its focus. In this case, a calibration curve need to be included in every experiment, consisting of at the least six serial dilutions of the in vitro−transcribed RNA https://www.facebook.com/NewTechFind/posts/pfbid02D8PWoz3PKpNehipTaRC5n74wEXHvDSQb8awWaNSbUXvSshqGwpLKrFqJjLRoVaawl